OBJECTIVE To describe the procedures for handling and restraint of mice used within UQBR facilities. Mice were separated into three groups: a gently handled condition, an aggressively handled condition, and a control condition (Table 1). Only open one cage of mice at a time. To reduce background variation and maximise welfare, methods that minimise handling stress should be developed and used wherever possible. cleaning) and research procedures (e.g. and J.T.). Introduction. Sharp iris scissors must be used. Mice must be approximately 7 days old at the time of the biopsy. Handling of Mice, Mouse Strains, Mouse Breeding Basics . Types of Breeding Systems, Setting up Matings, Mating Chimeras for Germline Transmission, Timed Pregnancies/ Plugging . However, many of the natural pathogens of these laboratory animals may alter host physiology, rendering the host unsuitable for many experimental uses. Adult mice are picked up by the tail base by compressing the base of the tail between the thumb and forefinger and … Handling of pregnant C57BL/6J mice (twice daily for 5 min throughout gestation) resulted in increased size of litter, decreased length of gestation, and decreased weight of offspring. Handling and Restraint of Mice. He also developed C57BL/10, C57BR, C57BL/6, and C57L. NB: The use of (*) indicates this statement is dependent on the facility procedures NB: The use of (**) indicates this statement is dependent on AEC Approvals II. 4.5.1.3. Check Pages 1 - 50 of of-mice-and-men full text in the flip PDF version. Skip to Article Content; Skip to Article Information ... View the article PDF and any associated supplements and figures for a period of 48 hours. A short video demonstrating humane restraint techniques in laboratory mice. Handling of Mice . Mice are social animals hence group-housing of mice is preferred over individual housing. Download PDF. Section 1 – Handling and Breeding of Mice. 3.7 Handling 42 Section 2, Chapter 2: Mice, rats, gerbils, hamsters and guinea pigs 43 – 47 1 Mice 43 1.1 Accommodation specifications 43 2 Rats 44 2.1 Accommodation specifications 44 3 Gerbils 45 3.1 Accommodation specifications 45 4 Hamsters 46 4.1 Accommodation specifications 46 5 Guinea pigs 47 5.1 Accommodation specifications 47 Using proper animal handling techniques can reduce pain and distress and therefore, increase animal welfare.Appropriate animal handling methods require training, skill, and behavioral knowledge of the species you are working with. RDI #12492 is a high fat diet. In addition, this treatment had differential effects upon the behavior of male and female offspring when they were tested at … Mice. mice and had began inbreeding mice with coat colors of dilute (d), brown (b), and non-agouti (a). handling mice prior to Morris water maze experi-ments. Download of-mice-and-men full text PDF for free. PROCUREMENT Guideline 11 The health status of the incoming mice should be reviewed before the animals are shipped. The mice of the non-handling groups (M-NH and E-NH) were handled only when necessary (e.g. Free PDF. The tail should be grasped The . SUMMARY Laboratory mice, rats, and rabbits may harbor a variety of viral, bacterial, parasitic, and fungal agents. Therefore, group-housed mice are frequently separated for welfare reasons. Animal handling describes how people respond to and interact with animals within their environment¹. Medical Center, and Department of Bites from rats are uncommon and will typically only occur if the animal is stressed or in pain. Directory of Research Offices. To initially restrain a rat, the handler should gently grasp it around the shoulders. Upon arrival, C57BL/6NTac mice are placed on an irradiated, sterility tested, high fat diet (RDI #12492). Empathy is thought to be unique to higher primates, possibly to humans alone. Routine laboratory animal handling has profound effects on their anxiety and stress responses, but little is known about the impact of handling method. Hypertension and Impaired Glycine Handling in Mice Lacking the Orphan Transporter XT2. Avoid handling mice whenever possible; animals can be observed through the cage. We found that picking up mice by the tail induced aversion and high anxiety, whereas use of tunnels or open hand led to voluntary approach, low anxiety and acceptance of physical restraint. Recent evidence has shown that handling mice by a familiar tunnel that is present in their home cage can … mice as closely as possible, and must receive prior Animal Ethics Committee approval. The package creates multiple imputations (replacement values) for multivariate missing data. However, aggression in group-housed male mice under laboratory housing conditions is a well-known problem leading to serious health issues, including injury or death. The feed is 35% fat by weight and 60% fat by kcal percent. National Academy of Sciences is a private, nonprofit, self-perpetuating society of distinguished scholars engaged in scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare. Office of the Senior Vice President for Research 304 Old Main University Park, Pennsylvania 16802 OSVPR Phone: 814-863-9580 Email: osvpr@psu.edu. We report the modulation of pain sensitivity in mice produced solely by exposure to their cagemates, but not to strangers, in pain. 8 Pages. Frequently, these organisms cause no overt signs of disease. Handling is a well-known source of stress to laboratory animals and can affect variability of results and even compromise animal welfare. A government review has found widespread uptake of new and ‘less aversive’ methods for handling mice in laboratories. In an experimental model of IgA nephropathy induced in mice by chronic immunization with dextran, we tested the hypothesis that a defect in the hepatic handling of IgA could be an important determinant in the deposition of IgA in the mesangium. 5.6.2. Mice tested in dyads and given an identical noxious stimulus displayed increased pain behaviors with statistically greater co-occurrence, effects dependent on visual observation. Abstract. Genetically Modified, Transgenic and Knockout Mice 1.6.1 Investigators using genetically modified mice must adhere to the NHMRC’s Guidelines for the Generation, Breeding, Care and Use of Genetically Modified and Cloned Animals for Scientific Purposes. 4.5.1.4. SAFETY 1. PDF. The method is based on Fully Conditional Specification, where each incomplete variable is imputed by a separate model. The mice were handled over Ngaire Dennison, president of the Laboratory Animals Veterinary Association, told delegates at the association’s conference last month that the traditional method for moving mice – involving picking them up by the tail – appears to be on the wane. Within specie, particular stocks or strains of animals may have distinctive behavioral responses. The standard method of handling laboratory mice often involves initially picking mice … Mice are maintained on the diet up to 26 weeks of age and are housed 10 per cage in the US and 5 per cage in Europe. Handling. Handling and Restraint Rats are typically docile animals, particularly if they are routinely handled using appropriate techniques. injections). Mice have been the most widely used laboratory animals for disease, behaviour, and pharmacology studies over the past century 1.To successfully transfer the results obtained in mice to human studies, it is necessary to determine appropriate treatment and handling methods for experimental mice since handling of normal mice may affect the obtained experimental results. 4.5.1.5. 4.5.1.2. Frequent handling makes them more docile while restrained. His dba strain became the DBA strain still popular today. Before opening the cage, spray the sides of the cage and lid with Accel®. Consider using handling forceps when possible. The MICE algorithm can impute mixes of continuous, binary, unordered categorical and ordered categorical data. PDF. Find more similar flip PDFs like of-mice-and-men full text. A rating scale for wildness and ease of handling laboratory mice: results for 21 inbred strains tested in two laboratories D. Wahlsten†,*, P. Metten‡ and J. C. Crabbe‡ †Department of Psychology and Centre for Neuroscience, University of Alberta, Edmonton AB, Canada,‡Portland Alcohol Research Center, V.A. The mice package implements a method to deal with missing data. the mice is strictly maintained. Handling mice: 5.6.1. Mice must be observed daily by trained personnel, with minimal disruption to the animals. No further biopsy can be performed. Handling stress is a well-recognised source of variation in animal studies that can also compromise the welfare of research animals. Care must be taken not to wet Mice were handled in the housing room under a lit biosafety cabinet by the same two experimenters (C.L. The Jackson Laboratory Handbook on Genetically Standardized Mice . To determine the impact of dietary state, ambient temperature, and anesthesia on the biodistribution of 18 F-FDG, groups of 3–6 mice each were studied under the experimental conditions summarized in Table 1.At the time of PET mice were 10–12 wk old with an average body weight ± SD of 24.2 ± 2.4 g. LAB_006 Handling and Restraint in Mice and Neonates I. 4.5.1.6. Our hypothesis was that if mice were handled in an escalated fashion, in accordance with the pro-tocol proposed by Deacon (1), the stress imposed by the investigator and the water maze would be reduced, resulting in fewer failures and reduced var-iation in naïve animals. Section 3.2 Personnel, p. 23 4. Only the most distal phalanx can be removed . 5.6.3. Mice are usually caught and lifted by the base of the tail. Of-mice-and-men full text was published by sandra.bunch on 2019-05-10. The importance of laboratory mice in experimental microbiology and biomedical research is indisputable, and their care and handling is a contributing factor to the quality of the science resulting from their use. Influence of Animal Preparation and Handling on Biodistribution of 18 F-FDG. during cage cleaning), whereas those of the handling groups (M-H and E-H) were handled and gentled each weekday for 30 60s (as suggested by Hirsja ¨rvi and Va ¨liaho 1995b), at variable Handling Missing Data in R with MICE Handling Missing Data in R with MICE Stef van Buuren1,2 1Methodology and Statistics, FSBS, Utrecht University 2Netherlands Organization for Applied Scientific Research TNO, Leiden Winnipeg, June 11, 2017 SvB Download Free PDF. mice can be acclimated to handling as adults.3 If acclimation to handling is not practical, it may help to handle the animal with a method that allows for movement between cages, without directly manipulating the animal in a way that it finds aversive. • Dr. Little established Jackson Labs in 1929 and was a recipient of The Nobel Prize. 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